If there is air leakage around the needle or loss of vacuum in the tube, replace the vacuum tube. Store tubes upright after collection. Turbid serum or plasma appears cloudy or milky. WebBeaumont Laboratorys tube chart shows the most commonly ordered tests with their associated specimen tube. Phos.Basic Met Pl. Laboratory procedures are performed ONLY upon a physician's written request. First, a blood specimen is drawn from a fasting patient. They are used for serum determinations in chemistry. Centrifuge tube. 0000002176 00000 n Laboratory Vernon, Olympia, Renton, Seattle, and Tacoma. Maintain at 2 - 8C before and during transport to the laboratory. Provide the patient, in advance, with appropriate collection instructions and information on fasting, diet, and medication restrictions when indicated for the specific test. 0000014084 00000 n Example: If a test requires 5 mL of EDTA whole blood, then a 2.5 mL purple tube cannot be used to procure the specimen. Stops are made at Sea-Tac Airport as needed. 4 74 (List Updated 02/11/2019) If blood is drawn by use of a syringe, transfer the specimen into the appropriate collection tube (s) by puncturing the rubber stopper with the syringe needle and allowing the blood to be drawn into the tube by the vacuum. Specimens that contain radioisotopes will be accepted for testing only when prior arrangements have been made and the specimens are clearly labeled so as to identify the contaminating isotope, the dose and compound administered to the patient, and the date of administration. (See appendices or individual tests for specific information.). Preparing the Patient. Different tests and biochemical assays require varying types of sample collection tubes. Do not shake tubes vigorously. When there is difficulty accessing a vein or when a vacuum tube fills too slowly due to a difficult venipuncture, damage to the red blood cells may result. If the specimen is watery and almost clear, it is probably saliva and is therefore inadequate for culturing. For lockbox instructions and Q&A, selectInstructions for Use. Garza, D., McBride, K.B. A successful launch starts with an informed strategy. Drug interference of notable clinical significance has been well-documented in the following instances. Used in some trace-metal testing and nortryptyline levels. While not always feasible, specimens should be collected before the administration of antimicrobial agents. The following is a list of tubes, additives present, and the state of those additives. Collect 5-10 grams of stool in a clean container. Longer tip segments can be cultured but will not be given a colony count. Centrifuge the specimen at the end of the waiting period in accordance with the manufacturer's instructions for speed. COLLECTION Always draw whole blood in an amount 2 times the required volume of serum required for a particular test. Different tests and biochemical assays require varying types of sample collection tubes. Also, use of a blood pressure cuff, in lieu of a tourniquet, will reduce trauma to fragile red blood cells. * T465 Clear Aliquot Tube Standard polypropylene tube for serum and plasma specimens. Collection Tubes Red Top contains no anticoagulant, for collection of serum samples. Authorization for laboratory work should include the name of the ordering physician and UPIN in addition to the name and address of the facility to which the results will be sent. Ferritin FTN SST Urea Nit, Blood BUN GRN FOL SSTVitamin B12 B12 GGT GGT SEROLOGY GRN INFECTIOUS DISEASE Blood Specimens: Chemistry and Hematology Preparing Serum, Combatting Modern Slavery and Human Trafficking Statement, Please examine specimen collection and transportation supplies to be sure they do not include, Label a specimen correctly and provide all pertinent information required on the test request form. Plasma Preparation Tube (PPT) with K2EDTA and Gel for Separation, spray-coated K2EDTA Tubes are used for whole blood hematology determinations and immunohematology testing. Our comprehensive laboratory services support clinical trials in countries around the world and are tailored to your product and requirements. Note:For chemistry profiles, 12- to 14-hour fasting specimens are recommended. It contains a detergent to lyse both red and white blood cells present in the sample, releasing any intracellular organisms. Allow collection site to dry after cleaning with the alcohol pad. There is a specific ratio of blood-to-liquid anticoagulant; if those ratios are not met, test results may be inaccurate. When multiple tests are ordered, sufficient samples or volume should be submitted in order to provide for the ideal recovery of organisms. Treat all biological material as material that is potentially hazardous as well as contaminated specimen collection supplies. For morning specimen collection, the laboratory recommends that the patient be required to fast from 6 PM on the previous evening. Most blood collection tubes contain an additive that either accelerates clotting of the blood (clot activator) or prevents the blood from clotting (anticoagulant). The minimum volume of blood for an adult is 5 mL in each bottle. Pay in 1 of 3 convenient ways: online, by email, or with a credit, debit, or health savings card. Different tests and biochemical assays require varying types of sample collection tubes. WebGlass tube with liquid ACD for use in blood bank studies, HLA phenotyping, DNA, paternity testing, etc. Causes and prevention are discussed under the section on hemolysis. Red Top contains no anticoagulant, for collection of serum samples. 0000808209 00000 n collection For lower volumes, submit refrigerat-ed urine in a sterile container without preservatives. Do not add fluid to the container. Transfer serum to a plastic vial unless otherwise specified. Quality test results start with correct specimen collection and handling. Minimum urine volume is 4 mL. It follows the principle, which is most commonly known as the "order of draw." Please do not send any specimens in glass containers unless specified elsewhere in this Laboratory Test Guide. It contains detailed instructions on requisitions, irreplaceable sample handling, collection procedures, infectious substances, and more. For UW Medicine only: If unable to draw directly into a blood culture bottle, draw into a SPS tube (not ACD) and the specimen will be transferred to bottles in the UW Microbiology laboratory. An SST tube should never be substituted as the gel barrier can interfere with analysis. Labs & Appointments Toggle Labs & Appointments, Billing & Insurance Toggle Billing & Insurance, Diseases & Conditions Toggle Diseases & Conditions, OnDemand Testing Toggle OnDemand Testing, Testing by Disease & Condition Toggle Testing by Disease & Condition, Testing & Services For Toggle Testing & Services For, Hospitals & Physician Systems Toggle Hospitals & Physician Systems, Managed Care Health Plans Toggle Managed Care Health Plans, Lab Data Integrations & Tools Toggle Lab Data Integrations & Tools, Employee Wellness & Testing Toggle Employee Wellness & Testing, Government & Education Toggle Government & Education, Environmental Testing Toggle Environmental Testing, Lab Testing & Research Toggle Lab Testing & Research, Data & Commercialization Toggle Data & Commercialization, Product & Molecule Testing Toggle Product & Molecule Testing, Partnering Solutions Toggle Partnering Solutions. Do NOT add the flocked swab to the transport media. It is of utmost importance to follow proper cleansing procedures for obtaining a clean catch or catheterized specimen. (See Quantity Not Sufficient.). Maintain at 2-8C before and during transport to the laboratory. Common blood collection tubes, their additives and laboratory No doctor's visit required. In cases of short draws or pediatric patients, it is recommended to use a pediatric 1.5 mL Isolator tube. After the tube has been filled with blood, immediately invert the tube gently several times to prevent coagulation. Do not allow it to freeze. Centrifuge for at least 15 minutes at 2200-2500 RPM. Swabs should be vigorously rubbed over any open lesions in mouth or throat. Break or cut the swab off into universal transport media (UTM). Provide patients with instructions for 24-hour urine collection(s). Each set contains one aerobic and one anaerobic bottle. The other is for a test that may require multiple blood specimens to be collected at several specific times. 0000020643 00000 n Do not overfill bottles. The accuracy of test results is dependent on the integrity of specimens. 0000013571 00000 n Laboratory Tube Collection It follows the principle, which is most commonly known as the "order of draw." WebSpecimen Collection Tubes Gold-top serum separator tube (SST) This tube contains a clot activator and serum gel separator used for various chemistry, serology, and immunology tests. The following is a list of tubes referred to throughout this Laboratory Test Guide. WebCOLLECTION TUBES. Type of specimen submitted, indicate serial and timed collection, Patient fasting conditions (if applicable), Interval and total volume of a timed urine collection, Source of the specimen if for Microbiology or Virology test(s), or other comments in "comments" box. *Note: After the tube has been filled with blood, immediately invert the tube gently several times to prevent coagulation. The composition of blood is altered after meals by nutrients being absorbed into the bloodstream. Separate plasma as soon as possible, preferably by centrifugation. If a specimen that is frozen needs to be put into the lockbox, the Frozen Specimen Keeper can be used maintain that frozen state. WebCheck the list of Light Protection Tests before packaging your specimen. Specimens should be collected using appropriate collection devices and should be tightly sealed to prevent leaks. Collection Tubes Shipping containers should be filled with dry ice to prevent samples from thawing in case of shipping delays. All primary specimen containers must be labeled with at least two identifiers at the time of collection. WebVacutainer. Do not substitute the yellow top (SPS) tube. Transport in sterile containers as quickly as possible. 0000001776 00000 n Other collection tubes may be rejected. Inhibits clotting by binding with calcium. Contains a spray-dried EDTA and separator gel. WebThere are four steps involved in obtaining a good quality specimen for testing: (1) preparation of the patient, (2) collection of the specimen, (3) processing the specimen, and (4) storing and/or transporting the specimen. Aspirate the fluid using a syringe. Mark the outside of the container: REFRIGERATED MATERIAL - DO NOT FREEZE. After the blood has stopped flowing, withdraw the needle. A test tube, also known as a culture tube or sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top and closed at the bottom. WebBlood Collection Tubes and General Guidelines **NOTE: Our testing is validated for BD vacutainers. Visit the Test Directory to view our test-specific specimen handling guidelines. They also cause an increase in total serum thyroxine-binding globulin. Remove debris around the wound by gently wiping the area with cotton soaked in sterile saline or water (do not moisten the swab with fluid containing bacteriostatic substances or antibiotics). 0000020185 00000 n %PDF-1.4 % Reference intervals are most frequently based on specimens from this collection period. 0000148369 00000 n Log into MyQuest to book or reschedule a lab visit, view test results, and more. (List Updated 02/11/2019) If blood is drawn by use of a syringe, transfer the specimen into the appropriate collection tube (s) by puncturing the rubber stopper with the syringe needle and allowing the blood to be drawn into the tube by the vacuum. Access your Supply Storefront Account to see previous orders, shipping addresses, and more. Common blood collection tubes, their additives and laboratory Cell-Free DNA BCT stabilizes cell-free plasma DNA as well as preserves cellular genomic DNA present in nucleated blood cells and circulating epithelial cells (tumor cells) found in whole blood. Maintain at 4 C before and during transport to the laboratory. The downloadable PDF also contains a linked Table of Contents, so you can easily find the information you are looking for. Maintain at 2 - 8C before and during transport to the laboratory. Your doctor must have ordered your COVID-19 antibody testing or you must have purchased a test Contains potassium oxalate and sodium fluoride, powder. First morning specimen is best. In order to ensure the safety of our couriers, and to make our processing workflow as streamlined and trouble-free as possible, we ask that you follow these guidelines: Certain specimens must be shipped frozen to maintain the stability of the analyte. Collection Please visit our appointment scheduling page to make your appointment. There are two types of timed blood specimens: One is for a single blood specimen ordered to be drawn at a specific time. This will dissolve antigens on the tube walls. Laboratory tube collection is a process applied when withdrawing blood samples from patients before they get tested in the laboratory. control the site you are about to enter and accepts no responsibility for its content. Red The red bottle is less common it is used for biochemistry tests requiring serum which might be adversely affected by the separator gel used in the yellow bottle. Adult or Pediatric, Light Green This tube contains heparin and plasma gel separator and is used for many different tests. Collect in sterile container. Common blood collection tubes, their additives and laboratory Avoid hemolysis, as some tests are invalidated by hemolysis. Patients who consume these foods within the 24-hour period immediately preceding collection of a blood specimen may have temporarily elevated lipid levels, which may be manifested by cloudy or lipemic serum. When submitting specimens to the University of Washington Department of Laboratory Medicine, please complete a requisition order form and send it with the specimen. If it is not possible to avoid placing the specimen lockbox in direct sunlight, add 20F to the temperatures listed above to determine how many frozen gel packs to use. *Please contact Client Support Services office at 206.520.4600 or 1 800 713-5198 if you intend to use our courier service for non-departmental delivery. Contains sodium heparin, vapor-coated or solid disc. Labeled bags for shipments sent to Client Support Services will be provided on request. 0000520597 00000 n please consult the online Test Directory, or contact your local Quest laboratory. through QuestDirect. Most laboratory tests are performed on anticoagulated plasma, serum, or whole blood. Boston, Pearson Education, 2017, B. Keichle, F. So Youre Going to Collect a Blood Specimen: An Introduction to Phlebotomy, Fifteenth Edition, College of American Pathologists. 0000806074 00000 n For emergency assistance on evenings and weekends, call the Laboratory Medicine resident on call at the UWMC Paging Operator, 206.598.6190. If all the specimens do not fit, use a larger biohazard bag (. Glass tube contains 1.5 mL Acid Citrate Dextrose (ACD) Solution A liquid: trisodium citrate (22.0g/L), citric acid (8.0g/L), and dextrose (24.5g/L).

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